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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte; Embrapa Gado de Leite. |
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Data corrente: |
17/03/2014 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARAUJO, C. P.; OSÓRIO, A. L. A. R.; JORGE, K. S. G.; RAMOS, C. A. N.; S. FILHO, A. F.; VIDAL, C. E. S.; ROXO, E.; NISHIBE, c.; ALMEIDA, N. F.; F. JUNIOR, A. A.; SILVA, M. R.; B. NETO, J. D.; CERQUEIRA, V. D.; ZUMÁRRAGA, M.; ARAUJO, F. R. |
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Afiliação: |
MARCIO ROBERTO SILVA, CNPGL. |
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Título: |
Detection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Plos One, v. 9, n. 3, p. 1-6, 2014 |
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Idioma: |
Inglês
Português |
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Conteúdo: |
In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with NVL. However, nested-PCR detected a significantly higher number of positive animals than the culture in the group of animals exhibiting LCT with no previous records of CITT. The use of the nested-PCR assay to detect M. bovis in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis. MenosIn the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with ... Mostrar Tudo |
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Palavras-Chave: |
Bovine and bubaline tuberculosis; Nested-PCR system. |
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Thesaurus Nal: |
Mycobacterium abscessus. |
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Categoria do assunto: |
--
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/119848/1/ARAUJO-C.-P..pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/99300/1/pone.0091023-1..6-2014-Nested-PCR.pdf
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Marc: |
LEADER 02739naa a2200325 a 4500
001 2010358
005 2024-02-05
008 2014 bl uuuu u00u1 u #d
100 1 $aARAUJO, C. P.
245 $aDetection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1.$h[electronic resource]
260 $c2014
520 $aIn the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with NVL. However, nested-PCR detected a significantly higher number of positive animals than the culture in the group of animals exhibiting LCT with no previous records of CITT. The use of the nested-PCR assay to detect M. bovis in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis.
650 $aMycobacterium abscessus
653 $aBovine and bubaline tuberculosis
653 $aNested-PCR system
700 1 $aOSÓRIO, A. L. A. R.
700 1 $aJORGE, K. S. G.
700 1 $aRAMOS, C. A. N.
700 1 $aS. FILHO, A. F.
700 1 $aVIDAL, C. E. S.
700 1 $aROXO, E.
700 1 $aNISHIBE, c.
700 1 $aALMEIDA, N. F.
700 1 $aF. JUNIOR, A. A.
700 1 $aSILVA, M. R.
700 1 $aB. NETO, J. D.
700 1 $aCERQUEIRA, V. D.
700 1 $aZUMÁRRAGA, M.
700 1 $aARAUJO, F. R.
773 $tPlos One$gv. 9, n. 3, p. 1-6, 2014
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 42 | |
| 1. |  | AZEVEDO, C.; DUNISCH, O.; CALIRI, G.; REIS, N.; GASPAROTTO, L.; BAUCH, J.; LEEUWEN, J. van. Tree growth and wood quality of Ceiba pentandra (sumauma) grown of "terra firme" and "varzea" sites. In: GERMAN-BRAZILIAN WORKSHOP ON NEOTROPICAL ECOSYSTEMS, 2000, Hamburg. Programa and abstracts... Hamburg: University, 2000. p. 260.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 3. | | AZEVEDO, C. P. de; DÜNISCH, O.; CALIRI, G.; SOUSA, N. R.; GASPAROTTO, L.; BAUCH, J.; LEEUWEN, J. van. Tree growth and wood quality of Ceiba pentandra (sumaúma) grown of "terra firme" and "varzea" sites. In: GERMAN-BRAZILIAN WORKSHOP ON NEOTROPICAL ECOSYSTEMS. ACHIEVEMENTS AND PROSPECTS OF COOPERATIVE RESEARCH, 2000, Hamburg. Proceedings... Hamburg: SHIFT: MADAM: WAVES, 2002. p. 325.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 6. | | BAUCH, J.; DUNISCH, O.; GASPAROTTO, L.; NEVES, E. M.; LIMA, R. M. B.; BUENO, M. Investigations on suitable tree especies for the reforestation of degraded land areas in Central Amazonia. In: SHIFT WORKSHOP, 2., 1995, Cuiaba. Summaries of lectures and posters... Cuiaba: UMFT, 1995. p. 163.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 8. | | BAUCH, J.; DUNISCH, O.; SCHUSTER, F.; GASPAROTTO, L.; AZEVEDO, C. P. de. Comparative study on wood characteristics of Carapa guianensis Aubl. from two platantions and a natural site in Central Amazonia. In: BAUCH, J.; DUNISCH, O.; GASPAROTTO, L. (Ed.). Investigations on tree species suitable for the recultivation of degraded land areas in Central Amazonia. Mitteilungen der Bundesforschungsanstalt für Forst- und Holzwirtschaft, n. 193, p. 97-112, Feb. 1999.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - C |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 9. | | BAUCH, J.; DÜNISCH, O.; SCHUSTER, F.; NEVES, E. Comparative study of wood characteristics of Carapa guianensis Aubl. from two plantations and a natural site in Central Amazonia. In: SYMPOSIUM TROPISCHE NUTZPFLANZEN: BIOLOGIE, OKOLOGIE, OKONOMIE, 2., 1997, Hamburg. Abstracts der Vortrage und Poster... Hamburg: University, 1997. p. 40.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 13. | | DUNISCH, O.; BAUCH, J.; GASPAROTTO, L.; NEVES, E.; AZEVEDO, C. de; LIMA, R. de; MORAIS, R. de. Environment-tree growth relationships of plantation grown tropical tree species as a basis for sustainable timber production in mixed culture systems in Central Amazonia. In: SHIFT - WORKSHOP, 3., 1998, Manaus. Proceedings... Bonn: BMBF, 1998. p. 399-411.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 14. | | DUNISCH, O.; BAUCH, J.; GASPAROTTO, L.; NEVES, E.; AZEVEDO, C. de; LIMA, R. de; MORAIS, R. de. Environment-tree growth relationships of plantation grown tropical tree species as a basis for sustainable timber production in mixed culture systems in central Amazonia. In: SHIFT-WORKSHOP, 3., 1998, Manaus. Program, abstracts of presentations and posters... Hamburg: GKSS, 1998. p. P23.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 17. | | DUNISCH, O.; BAUCH, J.; MULLER, M.; SCHWARZ, T.; POLACZCK, S.; GASPAROTTO, L.; NEVES, E.; AZEVEDO, C. de; LIMA, R. de; MORAIS, R. de. Investigations on tree species suitable for the recultivation of degraded land areas in Central Amazonia. In: SHIFT-WORKSHOP, 3., 1998, Manaus. Program, abstracts of presentations and posters... Hamburg: GKSS, 1998. p. C22.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 18. | | DÜNISCH, O.; BAUCH, J.; MÜLLER, M.; SCHWARZ, T.; POLACZEK, S.; GASPAROTTO, L.; NEVES, E.; AZEVEDO, C. de; LIMA, R. de; MORAIS, R. de. Investigations on tree species suitable for the recultivation of degraded land areas in Central Amazonia. In: SYMPOSIUM TROPISCHE NUTZPFLANZEN: BIOLOGIE, OKOLOGIE, OKONOMIE, 2., 1997, Hamburg. Abstracts der Vortrage und Poster... Hamburg: University, 1997. p. 52.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 19. | | DÜNISCH, O.; BAUCH, J.; SCHWARZ, T.; GASPAROTTO, L.; NEVES, E. Mineral element supply of plantation grown tropical trees species in Central Amazonia. In: SYMPOSIUM TROPISCHE NUTZPFLANZEN: BIOLOGIE, OKOLOGIE, OKONOMIE, 2., 1997, Hamburg. Abstracts der Vortrage und Poster... Hamburg: University, 1997. p. 22.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| Registros recuperados : 42 | |
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